Scientific training at the University of Pisa in Italy

During 28 June – 4 July I spent the training days at the University of Pisa.

I visited the laboratory of Prof. Chiara Gabelli and Prof. Vittoria Raffa where I learnt how to perform a gene editing protocol based on CRISPR-Cas9 technique, applied to human cancer cells as well as to the teleost zebrafish.

I prepared ribonucleoprotein complexes (RNP). I performed transfection of RNP and after I analyzed cell internalization of RNP using immunofluorescence. I learnt basic technique employing zebrafish including adults fish crossing, embryo collection, microinjection of RNP in embryos in 1-cell stage. In addition, I performed genomic DNA extraction from embryo and I gained basic knowledge in data analysis of High Resolution Melting technique. I received a valuable training on the new and very potent techniques.

Visiting the University of Pisa and being welcomed by the very hospitable people working there was a wonderful experience for me. In the broader perspective, the mobility can initiate the establishment of the research collaboration and staff exchange between University of Gdańsk and University of Pisa.

Alicja Dziadosz