During 28 June – 4 July I spent the training days at the University of Pisa.
I visited the laboratory of Prof. Chiara Gabelli and Prof. Vittoria Raffa where I learnt how to perform a gene editing protocol based on CRISPR-Cas9 technique, applied to human cancer cells as well as to the teleost zebrafish.
I prepared ribonucleoprotein complexes (RNP). I performed transfection of RNP and after I analyzed cell internalization of RNP using immunofluorescence. I learnt basic technique employing zebrafish including adults fish crossing, embryo collection, microinjection of RNP in embryos in 1-cell stage. In addition, I performed genomic DNA extraction from embryo and I gained basic knowledge in data analysis of High Resolution Melting technique. I received a valuable training on the new and very potent techniques.
Visiting the University of Pisa and being welcomed by the very hospitable people working there was a wonderful experience for me. In the broader perspective, the mobility can initiate the establishment of the research collaboration and staff exchange between University of Gdańsk and University of Pisa.