As part of the CANVAS project, a group of scientists from ICCVS: dr Elżbieta Chruściel, Martyna Muszczek Ines Papak and Łukasz Arcimowicz participated in 3D cell culture training organised by our project partner – RealResearch in Kraków, from 12.04.2023 until 23.04.2023. During our stay, we had a great opportunity to learn more about 3D cell culture from experts as, the CEO of Real Research – dr Marcin Krzykawski and the scientists working in his team: dr Krzysztof Klimkiewicz and dr David Earnshaw. During this time, we have learnt how to work with a 3D cell culture model based on LifeGel – a unique hydrogel developed by RealResearch. We learnt how to apply it for establishing primary cell cultures using clinical samples from the patients. Incredibly, after only a few days of training, we could already see 3D structures of the established primary cell lines forming. Obtained cultures allowed us to experiment a bit more with assays that can be performed on LifeGel – we performed metabolic activity assays, viability assays and fluorescent staining. The training allowed us to work confidently with LifeGel in ICCVS laboratories in Gdańsk, enabling us to continue to work on the CANVAS project. The training also presented a great opportunity for us to discuss research ideas and acquaint ourselves with colleagues from Kraków, and thanks to Marcin’s experience, it was a remarkable lesson on how to start and develop a scientific startup company. RealResearch Team also organised events that allowed for integration between scientists from ICCVS and the hosts. We had an amazing evening of axe-throwing and virtual reality games! During our free time, we explored the breathtaking, historical capital of Poland.